Mass spectrometry is a powerful analytical technique used to quantify known materials, to identify unknown compounds within a sample, and to elucidate the structure and chemical properties of different molecules. It allows precise determination of the molecular mass of peptides as well as their sequences. Mass spectrometry is used to confirm the identify of unknowns, such as illegal drugs. Detector System: For detecting the ions and recording the relative abundance of each of the resolved ionic species. The ions are separated in the mass spectrometer according to their mass-to-charge ratio, and are detected in proportion to their abundance. <> A mass spectrum of the molecule is thus produced. Keck Biomedical Mass Spectrometry Lab Moore Health Sciences Library Rooms 1335 & 1337 May 18, 2010 SimGlycan® predicts the structure of glycans and glycopeptides from the MS/MS data acquired by mass spectrometry, facilitating glycosylation and post translational modification studies. London WC1R 4HQ. Looks like you’ve clipped this slide to already. Oligonucleotides (DNA or RNA), are linear polymers of nucleotides. Square %���� Many new strategies for mass-spectrometry-based analyses of lipids have been developed. This molecular ion undergoes fragmentation. This information can very well be used for protein identification, de novo sequencing, and identification of post-translational modifications. Analyzer Resolutiona) Mass accuracy Full … It is used for determining masses of particles, for determining the elemental composition of a Ravishankar shukla university, Raipur, No public clipboards found for this slide. Sample ionization 17. registered in England (Company No 02017289) with its registered office at 26 Red Lion Mass spectrometry plays an important role in identifying these modifications and determining their structure as well as their position in the oligonucleotide. It not only allows determination of the molecular weight of oligonucleotides, but also in a direct or indirect manner, the determination of their sequences. endstream Entire OCR A-Level Chemistry Course Powerpoint, NEW AQA GCSE (2016) Chemistry - 'Rates of Reaction' lessons, OCR A Level Chemistry Year 2/A2 Revision Posters, OCR A Level Chemistry Module 5 Revision Posters. Figure: Components of a Mass Spectrometer, customer service software technical support. The first step in the mass spectrometric analysis of compounds is the production of gas phase ions of the compound, basically by electron ionization. If you continue browsing the site, you agree to the use of cookies on this website. It depends upon chemical reactions in the gas phase in which sample molecules are consumed during the formation of ionic and neutral species. Conditions. M.pharm 1st sem stream SimLipid can also annotate mass spectra with the lipid structures identified using abbreviations. endobj Spectroscopic data is often represented by the spectrum. Lipidomics, a major part of metabolomics, constitutes the detailed analysis and global characterization, both spatial and temporal, of the structure and function of lipids (the lipidome) within a living system. If you wish to opt out, please close your SlideShare account. of pharmaceutical chemistry. 4 1 Introduction to Mass Spectrometry, a Tutorial Table 1.1 Characteristicsandfeaturesofdifferentmassanalyzers. The development of desorption ionization methods based on the emission of pre-existing ions such as plasma desorption (PD), fast atom bombardment (FAB) or laser desorption (LD), allowed the application of mass spectrometry for analyzing complex biomolecules. <> It involves the determination of additional components as a consequence of the isomeric nature of monosaccharides and their capacity to form linear or branched oligosaccharides. SimLipid is an innovative lipid characterization tool which enables structural elucidation of unknown lipids using MS/MS data. See our User Agreement and Privacy Policy. Introduction Mass spectrometry (MS) is an analytical chemistry technique that helps identify the amount and type of chemicals present in a sample by measuring the mass-to-charge ratio and abundance of gas-phase ions. In the spectrum of a pure compound, the molecular ion, if present, appears at the highest value of m/z (followed by ions containing heavier isotopes) and gives the molecular mass of the compound. Learn more. Submitted by : Mass spectrometry “fingerprints” the components, so that they can be matched to existing known spectra . Mass spectrometry is fast becoming an indispensable field for analyzing biomolecules. Till the1970s, the only analytical techniques which provided similar information were electrophoretic, chromatographic or ultracentrifugation methods. Seminar presentation The software also supports multi stage mass spectrometry data analysis which enables structural elucidation and identification of fragmentation pathways. <> Created: Feb 11, 2013| Updated: Apr 10, 2020, A powerpoint and worksheet introducing students to The Mass Spectrometer AS AQA Chemistry. A mass spectrometer generates multiple ions from the sample under investigation, it then separates them according to their specific mass-to-charge ratio (m/z), and then records the relative abundance of each ion type. INTRODUCTION TO MASS SPECTROSCOPY Mass spectrometry (MS) is an analytical chemistry technique that helps identify the amount and type of chemicals present in a sample by measuring the mass-to- charge ratio and abundance of gas-phase ions.